Effects of Human and Porcine Adipose Extracellular Matrices Decellularized by Enzymatic or Chemical Methods on Macrophage Polarization and Immunocompetence

The decellularized extracellular matrix (ECM) obtained from human and porcine adipose tissue (AT) is currently used to prepare regenerative medicine bio-scaffolds. However, the influence of these natural biomaterials on host immune response not yet deeply understood. Since macrophages play a key role in inflammation/healing processes due their high functional plasticity between M1 M2 phenotypes, evaluation ECM mandatory. It also necessary analyze immunocompetence after contact with materials assess possible infection scenario. In this work, we studied effect four matrices (DAMs) AT by enzymatic or chemical methods macrophage phenotypes fungal phagocytosis. First, thorough biochemical characterization quantification remnant DNA, lipids, proteins was performed, thus indicating efficiency reliability both methods. proteomic analysis evidenced that some are differentially preserved depending origin decellularization method employed. After exposure DAMs, specific markers proinflammatory anti-inflammatory were analyzed. Porcine DAMs favor phenotype, independently Finally, sensitive phagocytosis assay allowed us relate capability certain DAMs. results study highlight close relationship composition macrophage’s role.